Recommendations for testing your cell line

The STR genotype of a cell line can evolve over multiple passages. We therefore recommend regular genotyping of your cell lines to monitor any change.

 When a new cell line is established or acquired: 

Authentication of an early passage (first week of culture)
Before freezing

As a standard quality control measure:

When beginning a new series of experiments
Once every two months

Before publication

When cell line performance is inconsistent or results are unexpected

To establish a genetic profile for a new or unique cell line. [1]





[1] FDA. General Requirements for Laboratory Controls. 21 CFR 211.160 and 21 CFR 610.18.